Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Base Pairing

DNA Base Pairing

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,

DNA Base Pairing

DNA Base Pairing

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,

DNA Topoisomerases

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. Type I...

DNA Helicases

DNA Helicases

DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...

Angle of Twist - Elastic Range

Angle of Twist - Elastic Range

Consider a cylindrical shaft with a length denoted by L and a consistent cross-sectional radius referred to as r. This shaft undergoes a torque at the free end. The highest shearing strain within the shaft is directly proportional to the twist angle and the radial distance from the shaft axis. When the shaft behaves elastically, this shearing strain can be articulated using variables such as the applied torque, radial distance, the polar moment of inertia, and the modulus of rigidity. By...

The DNA Helix

The DNA Helix

Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mitigation of renal toxicity induced by paraquat using ferulic acid: Role of inflammatory pathways.

Avicenna journal of phytomedicine·2026

Same author

Targeted exercise improves functional movement performance and alters movement screening relationships in females with flexible flatfeet.

Scientific reports·2025

Same author

Ganoderma lucidum mitigates oxidative stress and apoptosis in chlorpyrifos‑induced testicular toxicity in male rats.

Reproductive biology·2025

Same author

Systemic administration of gold nanoparticles attenuates anxiety-related behaviors and enhances spatial learning performance in rats.

IBRO neuroscience reports·2025

Same author

Gastroprotective and Antioxidant Properties of Linalyl Acetate in Ethanol-Induced Gastric Ulcer in Rats.

Advanced biomedical research·2025

Same author

The reuniens nucleus of the thalamus facilitates hippocampo-cortical dialogue during sleep.

eLife·2025

Search research articles

Related Experiment Video

Updated: Jun 25, 2026

Magnetic Tweezers for the Measurement of Twist and Torque

Magnetic Tweezers for the Measurement of Twist and Torque

Published on: May 19, 2014

Twist-stretch correlation of DNA.

Maryam Ghorbani¹, Farshid Mohammad-Rafiee

¹Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan 45195, P.O. Box 45195-1159, Iran.

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics

|March 5, 2009

Summary

We developed an elastic model for B-form DNA to analyze twist-stretch coupling. Our findings reveal a non-monotonic relationship, showing positive correlation for small distortions, aligning with experimental data.

Area of Science:

Biophysics
Molecular Biology
Computational Chemistry

Background:

Understanding DNA mechanics is crucial for molecular biology.
The relationship between DNA twisting and stretching is complex.
Previous models often simplify DNA's elastic properties.

Purpose of the Study:

To investigate the elastic twist-stretch coupling in B-form DNA under tension.
To develop a variable-radius elastic model for DNA.
To explore the correlation between DNA twisting and stretching dynamics.

Main Methods:

Developed a novel elastic model for B-form DNA.
Incorporated variable radius and bond energy changes (hydrogen and covalent).
Analyzed two primary strain variables during deformation.

More Related Videos

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Published on: October 13, 2011

Studying DNA Looping by Single-Molecule FRET

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

Related Experiment Videos

Last Updated: Jun 25, 2026

Magnetic Tweezers for the Measurement of Twist and Torque

Magnetic Tweezers for the Measurement of Twist and Torque

Published on: May 19, 2014

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Published on: October 13, 2011

Studying DNA Looping by Single-Molecule FRET

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

Main Results:

The twist-stretch coupling can be positive or negative, dependent on elastic constants.
DNA's twist-stretch coupling exhibits non-monotonic behavior.
A positive correlation between DNA twisting and stretching was observed for small distortions, contradicting initial intuition.

Conclusions:

The elastic model accurately captures complex DNA mechanical behavior.
The findings are consistent with recent experimental observations of DNA elasticity.
This model provides new insights into DNA's response to mechanical stress.