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Updated: Jun 25, 2026

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer
Published on: April 19, 2021
Dynamical transition, hydrophobic interface, and the temperature dependence of electrostatic fluctuations in proteins
David N Lebard1, Dmitry V Matyushov
1Center for Biological Physics, Arizona State University, P.O. Box 871604, Tempe, Arizona 85287-1604, USA.
Abstract:
Molecular dynamics simulations have revealed a dramatic increase, with increasing temperature, of the amplitude of electrostatic fluctuations caused by water at the active site of metalloprotein plastocyanin. The increased breadth of electrostatic fluctuations, expressed in terms of the reorganization energy of changing the redox state of the protein, is related to the formation of the hydrophobic protein-water interface, allowing large-amplitude collective fluctuations of the water density in the protein's first solvation shell. On top of the monotonic increase of the reorganization energy with increasing temperature, we have observed a spike at approximately 220 K also accompanied by a significant slowing of the exponential collective Stokes shift dynamics. In contrast to the local density fluctuations of the hydration-shell waters, these spikes might be related to the global property of the water solvent crossing the Widom line or undergoing a weak first-order transition.
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