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Updated: Jun 25, 2026
Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
Published on: August 11, 2018
[Studies on isolation, purification and antibacterial activities of beta-defensin in Yak neutrophils]
Kai-zhong Dong1, Si-jiu Yu, Ju-tian Yang
1College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China. dkz@xbmu.edu.cn
Aim:
To isolate and purify beta-defensin from the neutrophils of Yak.
Methods:
The method of percoll gradient centrifugation was employed to purify neutrophils from Yak peripheral blood. The crude extraction from Yak neutrophils was isolated respectively through a way of extraction by 50 mL/L acetic acid, acid soluble extract of Yak neutrophils was obtained. Then it was further purified by Bio-Gel P-10 Polyacrylamide gel filtration and RP-HPLC, and 9 out of 22 apices showed antibacterial activity. 9 apices showing antibacterial activity were selected to be analyzed by mass spectrograph.The antibacterial activities of the extracts from every step were analyzed by agarose radial diffusion assay.
Results:
The molecular weight of the purified Yak BNBD-1-3 was determined to be 4.2, 4.6 and 4.8 kDa by mass spectrograph. BNBD-1-3 from Yak neutrophils were able to effectively kill Escherichia coli, Staphylococcus aureus and Bacillus subtilis.
Conclusion:
Yak neutrophils have beta-defensin which have broad-spectrum antimicrobial activity, Yak defensin has an important part in innate immunity.
