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Establishing a corky ringspot disease plot for research purposes
H Mojtahedi1, R A Boydston, J M Crosslin
1USDA-ARS, 24106 N. Bunn Rd., Prosser, WA 99350 Washington State University, IAREC, 24106 N. Bunn Rd., Prosser, WA 99350. E. Riga is supported by the College of Agricultural, Human, and Natural Resource Sciences Agricultural Research Centre, PPNS No. 0452, Department of Plant Pathology and IAREC WSU-Prosser, Project No.WNP00542, Washington State University, Pullman, WA 99164-6430, USA.
Researchers established experimental corky ringspot disease (CRS) plots by introducing infected tobacco seedlings. This method successfully created CRS disease in potato fields for future research.
Area of Science:
- Plant Pathology
- Agricultural Science
- Virology
Background:
- Corky ringspot disease (CRS) is a significant potato ailment in the Pacific Northwest.
- The disease is caused by the tobacco rattle virus (TRV).
- TRV is primarily transmitted by the nematode Paratrichodorus allius.
Purpose of the Study:
- To develop a reliable method for establishing experimental plots with corky ringspot disease (CRS) in fields with no prior history of the disease.
- To create controlled environments for studying TRV transmission and CRS development in potatoes.
Main Methods:
- Inoculated 'Samsun NN' tobacco seedlings with viruliferous P. allius in a greenhouse setting.
- Transplanted these infected seedlings into field soil at a high density (3,000+ seedlings/ha).
- Monitored vector populations and virus presence using microscopy, bioassays on tobacco, and ELISA.
Main Results:
- The introduction of infected tobacco seedlings successfully established CRS disease in potato fields.
- After two years, 100% of soil samples from the established plot contained viruliferous P. allius.
- All five commercial potato cultivars tested in the second field expressed CRS symptoms, confirming the method's efficacy.
Conclusions:
- Transplanting greenhouse-inoculated tobacco seedlings with viruliferous P. allius is an effective method to establish experimental CRS disease plots.
- This technique provides a viable approach for creating controlled disease environments for potato research.
- The method ensures high infestation rates of the vector and successful disease expression in susceptible potato cultivars.
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