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Published on: February 12, 2022
Expression and subcellular localization of syntaxin 11 in human neutrophils
Li-Xin Xie1, Janis de la Iglesia-Vicente, Yun-Xiang Fang
1Department of Pharmacology, School of Pharmaceutical Science, Central South University, 410078 Changsha, China.
Summary
Human neutrophils express syntaxin 11, a protein linked to familial hemophagocytic lymphohistiocytosis (FHL). This finding suggests neutrophils may play a role in FHL pathology.
Area of Science:
- Immunology
- Cell Biology
Background:
- Familial hemophagocytic lymphohistiocytosis (FHL) is a severe hyperinflammatory syndrome.
- Syntaxin 11 mutations are a known cause of FHL.
- Neutrophils are key inflammatory cells.
Purpose of the Study:
- To investigate the expression and subcellular localization of syntaxin 11 in human neutrophils.
- To understand the role of neutrophils in FHL.
Main Methods:
- Utilized human peripheral blood neutrophils and HL-60 cells.
- Employed RT-PCR, Western blot, immunocytochemistry, and subcellular fractionation.
- Induced neutrophil differentiation in HL-60 cells.
Main Results:
- Syntaxin 11 mRNA and protein are expressed in human neutrophils.
- Syntaxin 11 expression increased during HL-60 cell differentiation.
- Syntaxin 11 is a membrane protein, primarily located in azurophilic granules, translocating to the plasma membrane upon activation.
Conclusions:
- Human neutrophils express syntaxin 11.
- Neutrophils may be involved in the pathology of familial hemophagocytic lymphohistiocytosis.
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