Related Experiment Video
Updated: Jun 25, 2026
Electroantennography-based Bio-hybrid Odor-detecting Drone using Silkmoth Antennae for Odor Source Localization
Published on: August 27, 2021
[Rapid determination on cocoonase contents in maxilla of four silkmoth breeds by dot blotting]
1College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China. houwew@163.com
Objective:
To determine the cocoonase content in maxilla of four silkmoth breeds of Bombyx mori and set up a dot blotting method to determine the content of the drug action components in Chinese medicines.
Methods:
The contents of cocoonase in maxilla of four silkworm breed were determined by dot blotting with rabbit antibody of anti-cocoonase as the first antibody. Blotting signals were analyzed by software of Quantity one.
Results:
Contents of cocoonase in maxilla of four silkmoth breeds were 6.41, 8.09, 7.62, and 1.88 microg/moth, respectively.
Conclusion:
The difference among four silkmoth breeds was significant in the content of maxilla cocoonase. Dot blotting, as a convenient and accurate method could be used for determination of the contents of drug action components in Chinese crude drugs similar to silkworm.
