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[Semen descurainiae inhibits CYP11B1, CYP11B2 and TGF-beta1 mRNA expression in left ventricular]
Juan Guo1, Chang-Xun Chen, Jun Du
1Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. guojuan338@yahoo.com.cn
Insights
Semen descurainiae effectively inhibits ventricular remodeling by reducing heart weight and down-regulating key gene expression. This suggests a therapeutic potential for cardiac conditions.
Area of Science:
- Cardiovascular Research
- Pharmacology
- Molecular Biology
Context:
- Cardiac remodeling is a significant pathological process following myocardial injury.
- Abdominal aortic banding (AAB) in rats is a model for inducing ventricular remodeling.
- CYP11B1, CYP11B2, and TGF-beta1 are implicated in cardiac hypertrophy and fibrosis.
Purpose:
- To evaluate the impact of Semen descurainiae and Captopril on cardiac remodeling markers.
- To investigate the effects on CYP11B1, CYP11B2, and TGF-beta1 mRNA expression in a rat model of AAB.
- To elucidate the molecular mechanisms underlying Semen descurainiae's potential cardioprotective effects.
Summary:
- Abdominal aortic banding (AAB) induced ventricular remodeling in rats, evidenced by increased left ventricle weight/body weight (LVW/BW) and heart weight/body weight (HW/BW) ratios.
- Treatment with Semen descurainiae significantly decreased LVW/BW and HW/BW ratios.
- Semen descurainiae administration led to a significant downregulation of CYP11B1, CYP11B2, and TGF-beta1 mRNA expression in the left ventricle.
Impact:
- Semen descurainiae demonstrates significant inhibitory effects on experimental ventricular remodeling.
- The cardioprotective mechanism of Semen descurainiae is associated with the attenuation of CYP11B1, CYP11B2, and TGF-beta1 mRNA expression.
- Inhibition of aldosterone key gene expression by Semen descurainiae may contribute to its therapeutic potential in managing cardiac remodeling.
Objective:
To investigate the effects of Semen descurainiae and Captopril on CYP11B1, CYP11B2 and TGF-beta1 mRNA expression of heart tissue in rats treated with Abdominal Aortic Banding.
Methods:
Ventricular remodeling was induced by abdominal aortic banding (AAB) in rats. After 30 days' treatment, the ratios of LVW/BW (left ventricle weight/body weight), HW/BW (heart weight/body weight) were calculated; Then the CYP11B, CYP11B2 and TGF-beta1 mRNA expression of left ventricle were detected by Real-time PCR, respectively.
Results:
The experimental data demonstrated that Semen descurainiae decreased the indexes of LVW/BW and HW/BW, down-regulated CYP11B, CYP11B2 and TGF-beta1 mRNA expression in left ventricle (P<0.05).
Conclusion:
Semen desceurainiae can significantly inhibit the experimental ventricular remodeling; the mechanism is related to its ability to attenuate the mRNA expression of CYP11B1, CYP11B2 and TGF-beta1 in left ventricle. The inhibition of aldosterone key gene expression by Semen descurainiae may contribute to its effect on restraint cardiac remodeling.
