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[Isolation-preparation and determination of chromones from Saposhnikovia divaricata]
Cun Zhang1, Yong-qing Xiao, Li Li
1Institute of Chinese Materia Madica, China Academy of Chinese Medicial Sciences, Beijing 100700, China.
Objective:
To compare the contents of chromones in the different isolation and purification procedures from Saposhnikovia divaricata.
Method:
The separation of six chromones was determined simultaneously by HPLC on a Kromasil C18 column at 35 degrees C with the methanol-0.1% phosphoric acid in gradient elution as the mobile phrase. The detection wavelength was set at 254 nm and the flow rate was 1.0 mL x min(-1).
Result:
All the obtained correlation coefficient of the six components was over 0.9995 and the average recoveries were not less than 97% (RSD <5%), respectively.
Conclusion:
The method is simple, accurate with good reproducibility. Results showed the better preparation procedure is water-decoction and ethanol-precipitation with resin separation and silica gel purification.
