Related Experiment Video
Updated: Jun 25, 2026
HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
[Determination of anthraquinone in Semen Cassiae from different regions by HPLC]
Yi Zhang1, Xiao-ping Huang, Dai-qun Weng
1Chongqing Academy of Chinese Materia Medcia, Chongqing 400065, China.
Objective:
To determine the content of 7 anthraquinones in Semen Cassiae.
Method:
A HPLC method was developed, with Inertsil ODS-3 column, acetonitrile and 0.1% H3PO4 solution as mobile phases in gradient elution. The detection wavelength wasset at 278 nm, and the flow rate was 0.8 mL x min(-1).
Result:
Recoveries of all 7 anthraquinones were between 95%-105%. The content of the anthraquinones in crude drug produced in different habitation were different.
Conclusion:
The method is convenient and accurate, which provides the foundation for the research of Semen Cassiae.
More Related Videos
10:13Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
Published on: November 12, 2016
10:30A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022