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Updated: Jun 25, 2026
07:29
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Lipid hydroperoxides in human plasma after ethanol consumption
Migiwa Asano1, Hideyuki Nushida, Junko Adachi
1Division of Legal Medicine, Department of Social Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. asano@med.kobe-u.ac.jp
Legal Medicine (Tokyo, Japan)
|March 6, 2009
Summary
Moderate alcohol consumption temporarily increases oxidative stress markers in healthy individuals. Total lipid hydroperoxides (LOOH) in plasma rise after drinking but are cleared quickly.
Area of Science:
- Biochemistry
- Toxicology
- Human Physiology
Background:
- Oxidative stress is a key factor in alcoholic liver disease development.
- Understanding the impact of moderate ethanol intake on oxidative stress is crucial.
Purpose of the Study:
- To quantify oxidative stress in healthy humans after moderate ethanol consumption.
- To investigate the relationship between ethanol intake and oxidative stress markers.
Main Methods:
- Blood samples collected from healthy volunteers pre- and post-ethanol consumption.
- Analysis of blood ethanol, total lipid hydroperoxides (LOOH), and cholesterol hydroperoxides.
- Aldehyde dehydrogenase 2 genotype determination.
Main Results:
- Plasma total lipid hydroperoxides (LOOH) significantly increased 1 hour after ethanol intake.
- Cholesterol hydroperoxides were not detected in plasma.
- No correlation was found between LOOH levels and blood ethanol concentration.
Conclusions:
- A single moderate dose of ethanol temporarily elevates plasma LOOH in healthy individuals.
- Elevated LOOH levels are rapidly cleared, suggesting efficient detoxification mechanisms.
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