Related Experiment Video
Updated: Jun 25, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
Published on: September 15, 2021
SpliceAid: a database of experimental RNA target motifs bound by splicing proteins in humans
Francesco Piva1, Matteo Giulietti, Linda Nocchi
1Institute of Biology and Genetics, Polytechnic University of Marche, 60131, Ancona, Italy. f.piva@univpm.it
Unlabelled:
The correct post-transcriptional RNA processing is finely regulated by RNA-binding proteins. Unfortunately, there is little experimental information on target RNA sequences of RNA-binding proteins and moreover such experimentally derived target sequences are annotated in a compact form by the score matrices that overestimate the number of possible recognized sequences. We carried out an exhaustive hand curated literature search to create a database, SpliceAid, collecting all the experimentally assessed target RNA sequences that are bound by splicing proteins in humans. We built a web resource, database driven, to easy query SpliceAid and give back the results by an accurate and dynamic graphic representation.
Availability:
SpliceAid database is freely accessible at http://www.introni.it/splicing.html.
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