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High-performance liquid chromatographic determination of ustiloxin A in forage rice silage
Shigeru Miyazaki1, Yuichi Matsumoto, Tsutomu Uchihara
1Safety Research Team, National Institute of Animal Health, NARO, Tsukuba, Ibaraki, Japan. s.miyazaki@affrc.go.jp
Abstract:
We describe a high-performance liquid chromatographic method for determining ustiloxin A, a mycotoxin produced by Ustilaginoidea virens, in forage rice silage. Lyophilized silage samples were ground and extracted with water. The extracts were purified by solid-phase extraction and subjected to high-performance liquid chromatography using an octadecylsilane-bonded column. Separated ustiloxin A was detected with ultraviolet (UV) absorption at 254 nm. The limit of quantitation for ustiloxin A in silage found to be 2.5 mg/kg. The present method can be used for routine monitoring of the contamination of ustiloxin A in forage rice silage.
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