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Age-related alterations in membrane lipid and protein interactions: Arrhenius studies of microsomal
Abstract:
Arrhenius plots of glucose-6-phosphatase (EC 3.1.3.9) activity in liver microsomes from 6-month-old rats (young) showed discontinuities at 39, 30, 20 and 12 degrees C and change in activation energy at 20 degrees C. The enzyme activity in kidney microsomes of young rats showed essentially the same thermal discontinuities. In liver microsomes of 24-month-old rats (old) the enzyme showed discontinuities at 38, 30, 24 and 16 degrees C but not at 12 degrees C and the activation energy changed at 24 degrees C. In kidney microsomes of old rats discontinuities were seen at 38, 31, 22, 16 and 12 degrees C and the activation energy was constant from 0 to 41 degrees C. These results indicate that the interactions of membrane components are altered with age.