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Related Concept Videos

Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Spermatogenesis01:41

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
Spermatogenesis01:22

Spermatogenesis

Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
Adult Stem Cells01:33

Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

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Related Experiment Video

Updated: Jun 25, 2026

Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines
12:26

Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines

Published on: February 25, 2013

The spermatogonial stem cell niche.

Dirk G de Rooij1

  • 1Center for Reproductive Medicine, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands. d.g.derooij@amc.uva.nl

Microscopy Research and Technique
|March 6, 2009
PubMed
Summary

The spermatogonial stem cell (SSC) niche, crucial for sperm production, is located near interstitial tissue. Factors from this tissue, like CSF1 and FSH, regulate SSC self-renewal and differentiation.

Area of Science:

  • Reproductive biology
  • Stem cell research
  • Spermatogenesis

Background:

  • Spermatogonial stem cells (SSCs) are essential for male fertility.
  • SSCs and their progeny reside in specific seminiferous tubule regions.
  • The location suggests a link between the SSC niche and surrounding interstitial tissue.

Purpose of the Study:

  • To investigate the relationship between the SSC niche and interstitial tissue.
  • To identify factors influencing SSC self-renewal versus differentiation.
  • To understand the spatial regulation of spermatogenesis.

Main Methods:

  • Analysis of SSC and progenitor cell distribution within seminiferous tubules.
  • Review of known growth factors produced by Sertoli and Leydig cells.

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Last Updated: Jun 25, 2026

Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines
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Extended Live Imaging of Female Drosophila melanogaster Germline Stem Cell Niches
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  • Hypothesizing the role of interstitial factors in niche determination.

    • Main Results:

    • SSCs and A(pr)/A(al) spermatogonia are concentrated near interstitial tissue.
    • Sertoli cells produce growth factors (GDNF, FGF2, activin A, BMP4, SCF) influencing SSCs.
    • Interstitial factors (CSF1, FSH) likely regulate SSC behavior via Leydig and Sertoli cells.

    Conclusions:

    • The SSC niche is spatially linked to interstitial tissue.
    • Interstitial factors, including CSF1 and FSH, play a key role in regulating SSC self-renewal and differentiation.
    • This spatial organization is critical for maintaining spermatogenesis.