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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

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Related Experiment Video

Updated: Jun 25, 2026

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
10:06

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

New frontiers in atomic force microscopy: analyzing interactions from single-molecules to cells.

Daniel J Müller1, Michael Krieg, David Alsteens

  • 1Biotechnology Center, Technische Universität Dresden, Dresden, Germany. daniel.mueller@biotec.tudresden.de

Current Opinion in Biotechnology
|March 7, 2009
PubMed
Summary

Atomic force microscopy (AFM) now analyzes biological interactions from single molecules to cells. Advanced AFM techniques reveal molecular details of cell adhesion, crucial for life science and biotech research.

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Last Updated: Jun 25, 2026

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
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Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
09:48

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

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Area of Science:

  • Biophysics
  • Cell Biology
  • Nanotechnology

Background:

  • Atomic force microscopy (AFM) was initially developed for surface imaging.
  • AFM has advanced into a versatile tool for molecular investigations in biological systems.
  • Understanding cell interactions is vital for life science and biotechnology.

Purpose of the Study:

  • To highlight the evolution of AFM into a multifunctional molecular toolkit.
  • To showcase AFM's application in investigating biological systems from single-molecules to whole cells.
  • To emphasize AFM's potential in understanding cell adhesion processes.

Main Methods:

  • Nanoscale imaging of individual cell chemical properties.
  • Single-molecule force spectroscopy for detecting and analyzing cell surface receptors.
  • Single-cell force spectroscopy for quantitative measurement of cellular interactions.

Main Results:

  • AFM enables detailed nanoscale imaging of cellular chemical properties.
  • Single-molecule force spectroscopy effectively detects and analyzes cell surface receptors.
  • Single-cell force spectroscopy provides quantitative insights into cellular interactions.

Conclusions:

  • Advanced AFM force spectroscopy modalities offer novel approaches to study cell adhesion.
  • These techniques are pivotal for unraveling the molecular mechanisms underlying cell adhesion.
  • AFM advancements address fundamental challenges in current life science and biotech research.