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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

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Related Experiment Video

Updated: Jun 25, 2026

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

Notch regulates cytolytic effector function in CD8+ T cells.

Ok Hyun Cho1, Hyun Mu Shin, Lucio Miele

  • 1Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|March 7, 2009
PubMed
Summary
This summary is machine-generated.

Notch1 signaling is crucial for CD8(+) T cell maturation into cytotoxic T lymphocytes (CTLs). This pathway directly regulates eomesodermin (EOMES), perforin, and granzyme B, key molecules for CTL function.

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Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
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Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

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Last Updated: Jun 25, 2026

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
09:57

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Published on: January 29, 2019

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
10:14

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

Published on: December 17, 2013

Area of Science:

  • Immunology
  • Cellular Biology
  • Molecular Biology

Background:

  • Naive CD8(+) T cells mature into effector cytotoxic T lymphocytes (CTLs), a vital part of adaptive immunity.
  • Eomesodermin (EOMES) is a transcriptional regulator essential for CTL development, controlling perforin and granzyme B expression.
  • The exact mechanisms governing CD8(+) effector cell maturation are not fully understood.

Purpose of the Study:

  • To investigate the role of Notch1 signaling in the regulation of CD8(+) T cell effector function.
  • To elucidate the molecular mechanisms by which Notch1 influences the expression of EOMES, perforin, and granzyme B.

Main Methods:

  • Biochemical and genetic abrogation of Notch signaling pathways.
  • Analysis of Notch1 binding to the promoter regions of EOMES, perforin, and granzyme B.
  • Assessment of effector molecule expression in CD8(+) T cells following Notch pathway manipulation.

Main Results:

  • Notch1 directly binds to the promoter regions of EOMES, perforin, and granzyme B.
  • Abrogation of Notch signaling significantly reduces the expression of EOMES, perforin, and granzyme B.
  • Notch activity is demonstrated to be essential for the expression of these key effector molecules.

Conclusions:

  • Notch1 signaling is a critical regulator of CD8(+) T cell effector maturation.
  • Notch1 directly controls the expression of EOMES, perforin, and granzyme B, thereby mediating CTL activity.
  • Understanding Notch1's role provides insights into adaptive immunity and potential therapeutic targets.