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Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

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Related Experiment Video

Updated: Jun 25, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

STIM1-independent T cell development and effector function in vivo.

Niklas Beyersdorf1, Attila Braun, Timo Vögtle

  • 1Institute for Virology and Immunobiology, Deutsche Forschungsgemeinschaft Research Center for Experimental Biomedicine, University of Würzburg, Germany.

Journal of Immunology (Baltimore, Md. : 1950)
|March 7, 2009
PubMed
Summary

Store-operated calcium entry (SOCE) regulated by STIM1 is vital for T cell proliferation. However, STIM1 deficiency did not impede T cell maturation or effector functions in this study.

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Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

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Published on: December 7, 2015

Area of Science:

  • Immunology
  • Cell Biology
  • Calcium Signaling

Background:

  • Store-operated calcium entry (SOCE) is critical for T cell function.
  • Stromal interaction molecule 1 (STIM1) is a key regulator of SOCE.

Purpose of the Study:

  • To investigate the role of STIM1-dependent SOCE in T cell physiology.
  • To determine the necessity of STIM1 for T cell maturation, proliferation, and effector functions.

Main Methods:

  • Generation of STIM1-deficient mice.
  • In vitro analysis of calcium flux in T cells.
  • In vivo studies including vaccination and adoptive T cell transfer.
  • Generation of bone marrow chimeric mice.

Main Results:

  • STIM1 deficiency abolished SOCE and antigen receptor-triggered calcium flux in T cells.
  • STIM1-deficient mice exhibited lymphoproliferative disease.
  • T cell maturation and regulatory T cell frequencies were normal.
  • Humoral immune responses and graft-versus-host disease induction were intact.

Conclusions:

  • STIM1-dependent SOCE is essential for T cell homeostasis and proliferation.
  • STIM1 is less critical for T cell differentiation and effector functions.
  • SOCE is not required for thymic T cell maturation or immune responses like vaccination.