[A comparative evaluation of the glycogen content in the peripheral blood of normal cattle and in chronic lymphoid...

Peripheral blood lymphocytes in cows of four groups (healthy, exposed to leucosis, those affected by leucosis at early or at developed stages) were tested for their glycogen contents by the two following methods: the semiquantitative PAS technique (Shabadash, 1947) and the quantitative cytofluorimetric variant of PAS reaction in the M. V. Kudriavtseva modification (1970). The results show no difference between the 1st and 2nd groups as concerns the number of leucocytes and the relation of the number of glycogen-positive cells to the share of lymphocytes in the peripheral blood. In the 3rd and 4th groups, the increase in glycogen in individual cells paralleled with the increase in the number of PAS-positive lymphocytes, according to the Shabadash method. On comparing the results of the study of glycogen contents in lymphocytes of healthy and leucosis suffering cows, the two methods used proved to coincide very well. Hence, both the methods may be used for the aims of diagnosis of hemoblastosis in farm animals.