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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
PCR01:32

PCR

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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

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In vitro Mutagenesis01:16

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Updated: Jun 25, 2026

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology
08:19

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Published on: May 28, 2020

Meloidogyne javanica Chorismate Mutase Transcript Expression Profile Using Real-Time Quantitative RT-PCR.

Janet E Painter, Kris N Lambert

    Journal of Nematology
    |March 7, 2009
    PubMed
    Summary

    The chorismate mutase-1 (Mj-cm-1) gene in Meloidogyne javanica shows peak expression early in parasitism. This suggests its product is crucial for nematode establishment during the infective second-stage juvenile (Inf-J2) to early feeding stages.

    Keywords:
    Meloidogyne javanicaMj-ba-1Mj-cm-1Taqman assaycDNAchorismate mutasedevelopmental profilemRNAnematodeparasitism genereal-time quantitative RT-PCRroot-knot nematodeshikimate pathwaytranscript

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    Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

    Published on: July 29, 2019

    Area of Science:

    • Plant-parasitic nematodes
    • Molecular biology
    • Gene expression analysis

    Background:

    • Meloidogyne javanica is a significant plant-parasitic nematode.
    • Esophageal gland secretions play a key role in nematode parasitism.
    • Understanding gene function during the lifecycle is crucial for developing control strategies.

    Purpose of the Study:

    • To determine the developmental expression profile of the chorismate mutase-1 (Mj-cm-1) gene in M. javanica.
    • To correlate Mj-cm-1 expression with specific lifecycle stages.
    • To infer the functional role of Mj-cm-1 during nematode parasitism.

    Main Methods:

    • Real-time quantitative RT-PCR was used to quantify Mj-cm-1 mRNA levels.
    • Six distinct lifecycle timepoints were analyzed: egg, infective second-stage juveniles (Inf-J2), and 2, 7, 14 days post-inoculation (pi), and adult.
    • Mj-javanica Beta-actin-1 (Mj-ba-1) served as the control gene.

    Main Results:

    • Mj-cm-1 mRNA levels exhibited a significant peak approximately 100-fold higher at 2 days pi compared to egg and Inf-J2 stages.
    • Mj-cm-1 expression was detected across all analyzed timepoints, including later stages (7-day pi, 14-day pi, adult).
    • Consistent Mj-ba-1 expression confirmed cDNA presence throughout the lifecycle.

    Conclusions:

    • The peak expression of Mj-cm-1 at 2 days pi suggests its product is functionally important early in the establishment of parasitism.
    • The localization of Mj-cm-1 mRNA in esophageal glands supports its role in secreting effector proteins.
    • This gene is a potential target for disrupting the early stages of M. javanica infection.