Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Four in One: Parallel Determination of Optical Activity and Optical Anisotropy from Single Plasmonic Nanostructures.

ACS nano·2026
Same author

Heterogeneous Reactivity of Palladium Nanoparticles Revealed by Wavelength-Resolved Interferometric Scattering.

Nano letters·2026
Same author

Peer Review and AI: Your (Human) Opinion Is What Matters.

ACS nano·2026
Same author

Kinetically Controlled Seed-Mediated Synthesis of Colloidal Copper Nanotetrahedra with Intricate Internal Structure.

Journal of the American Chemical Society·2025
Same author

The Next Ten Years of Nanochemistry: Summary of a Community Workshop on Key Challenges and Opportunities.

ACS nano·2025
Same author

Learning from Metal Nanocrystal Heterogeneity: A Need for Information-Rich and High-Throughput Single-Nanocrystal Measurements.

ACS nanoscience Au·2025

Related Experiment Video

Updated: Jun 25, 2026

Label-Free Surface-Enhanced Raman Scattering Bioanalysis Based on Au@Carbon Dot Nanoprobes
06:19

Label-Free Surface-Enhanced Raman Scattering Bioanalysis Based on Au@Carbon Dot Nanoprobes

Published on: June 9, 2023

Surface-enhanced Raman scattering (SERS) for probing internal cellular structure and dynamics.

Katherine A Willets1

  • 1Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX 78712, USA. kwillets@mail.utexas.edu

Analytical and Bioanalytical Chemistry
|March 7, 2009
PubMed
Summary

Surface-enhanced Raman scattering (SERS) offers molecular insights within cells. This review covers challenges and strategies for using SERS nanoparticles for intracellular analysis and understanding cellular dynamics.

More Related Videos

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

Related Experiment Videos

Last Updated: Jun 25, 2026

Label-Free Surface-Enhanced Raman Scattering Bioanalysis Based on Au@Carbon Dot Nanoprobes
06:19

Label-Free Surface-Enhanced Raman Scattering Bioanalysis Based on Au@Carbon Dot Nanoprobes

Published on: June 9, 2023

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

Area of Science:

  • Biophysics
  • Nanotechnology
  • Cell Biology

Background:

  • Surface-enhanced Raman scattering (SERS) provides molecular vibrational data from nanoparticles.
  • Intracellular analysis requires understanding molecular composition and dynamics.
  • SERS offers a potential route for in situ cellular investigation.

Purpose of the Study:

  • To review the application of SERS for intracellular analysis.
  • To highlight challenges and successes in applying SERS within living cells.
  • To discuss strategies for improving SERS specificity in biological environments.

Main Methods:

  • Review of existing literature on SERS applications in cellular environments.
  • Analysis of nanoparticle delivery methods into cells.
  • Discussion of spectral interpretation complexities in biological matrices.

Main Results:

  • SERS can provide valuable molecular information from within cells.
  • Challenges include nanoparticle internalization and complex spectral interpretation.
  • Strategies exist to enhance SERS specificity and data acquisition.

Conclusions:

  • Applying SERS inside living cells is feasible but challenging.
  • Overcoming delivery and interpretation hurdles is key for advancing intracellular SERS.
  • Further development is needed for routine in vivo SERS analysis.