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Updated: Jun 25, 2026

Sediment Core Extrusion Method at Millimeter Resolution Using a Calibrated, Threaded-rod
Published on: August 17, 2016
Enhanced resolution of sedimentation coefficient distribution profiles by extrapolation to infinite time
1Max Delbrück Center for Molecular Medicine, Robert Rössle Str. 10, 13125 Berlin, Germany. behlke@mdc-berlin.de
Abstract:
Sedimentation velocity experiments can be used to identify two or more independent non-interacting macromolecules, which differ in their size by only a few percent. The procedure requires the extrapolation of differential apparent sedimentation coefficient distributions obtained at different running time to t --> infinity and works because it eliminates or greatly reduces diffusion effects. Here, we present an improved time extrapolation function of sedimentation distribution profiles originally presented by Stafford (In: Harding, Rowe, Horton (eds.) Analytical ultracentrifugation in biochemistry and polymer science, 1992). We describe a computing procedure with the program LAMM: to analyze concentration profiles obtained by absorbance or interference optics that utilizes suitable smoothing methods for noisy data sets and present examples which include time invariant noises.
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