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Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jun 25, 2026

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

Pathologic phenotyping of mutant mice.

Roderick T Bronson1

  • 1Department of Biomedical Sciences, Pathology, Tufts University, Massachusetts, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 7, 2009
PubMed
Summary

Comprehensive necropsy and histopathology are cost-effective methods for analyzing knockout mouse phenotypes. Examining lesions in knockout mice can reveal insights into disease pathogenesis across species.

Area of Science:

  • Comparative Pathology
  • Genetically Modified Animal Models
  • Histopathology Techniques

Background:

  • Phenotypic analysis of knockout mice is crucial for understanding gene function.
  • Traditional methods can be time-consuming and expensive.
  • Existing literature on mouse lesions is extensive but requires efficient search strategies.

Purpose of the Study:

  • To outline the most effective and economical approach for knockout mouse phenotyping.
  • To detail methods for preparing mouse tissues for comprehensive histopathological examination.
  • To facilitate the investigation of lesion pathogenesis by linking mouse findings to broader biological contexts.

Main Methods:

  • Performing a thorough necropsy on knockout mice.
  • Systematic histopathological examination of all tissue slides.

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Last Updated: Jun 25, 2026

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  • Utilizing established and novel histological preparation techniques suitable for diverse lesions and ages.

    • Main Results:

    • A comprehensive necropsy and histopathology workflow enables efficient phenotype analysis.
    • Prepared slides allow for detailed study of lesions and comparison with existing literature.
    • The described methods are adaptable for various mouse models and observed pathologies.

    Conclusions:

    • Integrated necropsy and histopathology provide a cost-effective strategy for knockout mouse phenotyping.
    • This approach aids in identifying conserved lesion patterns and understanding disease mechanisms.
    • The described techniques enhance the utility of knockout mice as models for human diseases.