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Updated: Jun 25, 2026
Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)
Published on: April 21, 2019
[Extraction, isolation and immunological identification of allergens from Pseudosciaena crocea]
Rui Yang1, Haiqiang Wu, Zhigang Liu
1Allergy and Immunology Institute, Shenzhen University, Shenzhen 510860, China. yangruimail@yahoo.com.cn
Objective:
To extract, identify and purify the major allergens of Pseudosciaena crocea in order to evaluate the immunological activities.
Methods:
Pseudosciaena crocea proteins were extracted by Coca's buffer. Allergens of the Pseudosciaena crocea were identified by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western-Blotting. Major allergens were isolated by ion exchange chromatagraphy (IEC) and their immunological activities were evaluated by Western-Blotting with the serum of patients as 1st antibody.
Results:
The molecular weight of the Pseudosciaena crocea protein were between 8-116 kD. The molecular weight of the major allergens of Pseudosciaena crocea were 54, 29, 27, 14 kD. The concentration of allergens of Pseudosciaena crocea after isolated by IEC were highly improved and the immunological activities were kept.
Conclusion:
The allergens of Pseudosciaena crocea were detected and identified by immunological activity.
