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Updated: Jun 25, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Self-assembly of a four-helix bundle on a DNA quadruplex
Brooke A Rosenzweig1, Andrew D Hamilton
1Department of Chemistry, Yale University, P.O. Box 208017, New Haven, CT 06520-8107, USA.
Abstract:
Come together: A novel method for assembling monomers and controlling structure of a de novo helix bundle protein is described. A guanine (G)-rich oligodeoxynucleotide scaffold forms a hydrogen-bonded DNA quadruplex in the presence of potassium counterions, thereby inducing a helical structure and fourfold stoichiometry in conjugated, amphiphilic peptide sequences. The DNA scaffold shows potential for rapidly assembling designed proteins.
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