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Related Concept Videos

In vitro Mutagenesis01:16

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Related Experiment Video

Updated: Jun 25, 2026

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
07:18

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

A frequency-controlled random mutagenesis method for GC-rich genes.

Pengfu Liu1, Yuzhi Hong, Yongjun Lin

  • 1State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.

Analytical Biochemistry
|March 10, 2009
PubMed
Summary

This study introduces a new random mutagenesis technique combining sodium bisulfite modification and polymerase chain reaction (PCR). The method effectively introduces GC to AT substitutions, ideal for modifying GC-rich genes and aiding directed molecular evolution.

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Last Updated: Jun 25, 2026

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Area of Science:

  • Molecular Biology
  • Biotechnology

Background:

  • Random mutagenesis is crucial for understanding gene function and protein engineering.
  • Existing methods may have limitations for specific DNA types, such as GC-rich sequences.

Purpose of the Study:

  • To develop a novel random mutagenesis strategy.
  • To enhance the modification of GC-rich genes.
  • To provide a tool for directed molecular evolution.

Main Methods:

  • Combined sodium bisulfite modification with polymerase chain reaction (PCR).
  • Utilized GC to AT substitution for targeted mutagenesis.
  • Controlled mutation frequency by adjusting modification time.

Main Results:

  • The novel method efficiently introduced GC to AT substitutions.
  • Mutation efficiency was directly correlated with modification time.
  • Achieved desired and adequate random mutation frequencies, particularly in GC-rich genes.

Conclusions:

  • The developed method is effective for random mutagenesis, especially for GC-rich DNA.
  • This technique offers a powerful tool for directed molecular evolution.
  • Controllable mutation frequency allows for tailored genetic modifications.