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Simple and sensitive fluorometric sensing of malachite green with native double-stranded calf thymus DNA as sensing
1Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Shida Road No. 1, Xi'an 710062, PR China.
Abstract:
A novel fluorometric sensing of malachite green is proposed in this paper. The native double-stranded calf thymus DNA was used as sensing material. In the presence of native double-stranded calf thymus DNA, malachite green could interact with the DNA, which resulted in a strong fluorescence emission. The fluorescent intensity was linear with malachite green concentration in the range of 4.0 x 10(-10)-1.8 x 10(-7)g ml(-1) and the limit of detection was 2.0 x 10(-10)g ml(-1). Before fluorescence measurement, the only required operation is the mixing of two solutions. So, this method is rather simple and rapid. The method is very safe for the analyst. Furthermore, the mechanism for fluorescence enhancing of native double-stranded calf thymus DNA on MG was proposed based on a series of experiments. The results suggest that the interaction between MG and calf thymus DNA is intercalation in nature.
