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Isolation of Aleutian disease virus of mink in cell culture
Abstract:
Aleutian disease virus, the causative agent of a persistent infection in mink, was isolated in a continuous line of feline renal cells when the cultures were maintained at reduced temperature (31.8 degrees). After serial in vitro passage of the virus at this temperature it had an optimum replication temperature of 37 degrees. An immunofluorescence focus assay was found to be suitable for virus quantitation. The cultured virus reproduced Aleutian disease in mink, and the virus could be reisolated from the mink 10--180 days after inoculation. The properties of the virus suggest that it is a member of the parvovirus group.
Insights
Researchers successfully cultured Aleutian disease virus in feline cells at low temperatures, demonstrating its ability to cause disease in mink and suggesting it is a parvovirus.
Area of Science:
- Veterinary Virology
- Infectious Diseases
- Cell Culture Techniques
Background:
- Aleutian disease virus (ADV) causes persistent infections in mink.
- Understanding ADV replication is crucial for disease management.
- Previous isolation methods were limited.
Purpose of the Study:
- To establish an in vitro cell culture system for ADV.
- To characterize ADV replication and pathogenesis.
- To confirm the viral classification of ADV.
Main Methods:
- Isolation of ADV in feline renal cells at reduced temperature (31.8°C).
- Serial in vitro passage and temperature optimization for replication.
- Quantitation using immunofluorescence focus assay.
- Inoculation of mink to assess pathogenicity and reisolation.
Main Results:
- ADV was successfully isolated and propagated in feline renal cells.
- Optimal replication occurred at 37°C after initial low-temperature adaptation.
- Immunofluorescence assay proved effective for virus quantification.
- Inoculated mink developed Aleutian disease, with virus reisolation confirmed.
Conclusions:
- A viable cell culture system for ADV was established.
- The study confirmed ADV's pathogenic potential and provided a method for its study.
- Viral properties support classification within the parvovirus group.