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Updated: Jun 25, 2026

Metagenomic Analysis of Silage
Published on: January 13, 2017
Rapid identification of genes encoding DNA polymerases by function-based screening of metagenomic libraries derived
Carola Simon1, Judith Herath, Stephanie Rockstroh
1Abteilung Genomische und Angewandte Mikrobiologie, Institut für Mikrobiologie und Genetik der Georg-August-Universität, Grisebachstr. 8, 37077 Göttingen, Germany.
Abstract:
Small-insert and large-insert metagenomic libraries were constructed from glacial ice of the Northern Schneeferner, which is located on the Zugspitzplatt in Germany. Subsequently, these libraries were screened for the presence of DNA polymerase-encoding genes by complementation of an Escherichia coli polA mutant. Nine novel genes encoding complete DNA polymerase I proteins or domains typical of these proteins were recovered.
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