PCR-based method using propidium monoazide to distinguish viable from nonviable Bacillus subtilis spores
H Rawsthorne1, C N Dock, L A Jaykus
1Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, 400 Dan Allen Drive, Raleigh, NC 27695-7624, USA. Helen_Rawsthorne@NCSU.edu
Applied and Environmental Microbiology
|March 10, 2009
Abstract:
This paper describes a molecular-based method which is able to discriminate between viable and inactivated Bacillus subtilis spores by utilizing the DNA-intercalating dye propidium monoazide. The approach should be valuable in our attempt to employ molecular methods to streamline the evaluation of process validation using bacterial endospores.
