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[Contamination of fish tissue cell cultures by Mycoplasma]
Vestnik Akademii Meditsinskikh Nauk SSSR
|January 1, 1991
Abstract:
The inoculated and primary cell cultures of fish (carp, salmon, and sturgeon) have been studied. Acholeplasma typed as A. laidlawii in terms of its biochemical properties shown in inhibited metabolism has been isolated from 19 samples. The authors consider the source of Acholeplasma-induced contamination of the cell cultures under study.
Insights
Acholeplasma laidlawii contamination was identified in 19 fish cell cultures, impacting carp, salmon, and sturgeon. Researchers investigated the source of this microbial contamination in cell cultures.
Area of Science:
- Microbiology
- Cell Culture Technology
- Aquatic Animal Health
Context:
- Fish cell cultures are vital for research and diagnostics.
- Microbial contamination poses a significant threat to cell culture integrity.
- Acholeplasma species are known opportunistic contaminants.
Purpose:
- To identify the source of Acholeplasma contamination in fish cell cultures.
- To characterize the isolated Acholeplasma strain.
- To understand the implications of Acholeplasma contamination on fish cell metabolism.
Summary:
- Acholeplasma laidlawii was isolated from 19 inoculated and primary fish cell cultures (carp, salmon, sturgeon).
- Biochemical analysis confirmed the identity of Acholeplasma laidlawii, noting its effect on cellular metabolism.
- The study explores potential origins of Acholeplasma contamination within these aquatic cell culture systems.
Impact:
- Highlights the prevalence and impact of Acholeplasma contamination in fish cell lines.
- Provides insights into managing and preventing microbial contamination in sensitive cell cultures.
- Informs best practices for maintaining the quality and reliability of fish cell-based research.