Modulation of paracetamol and nefopam antinociception by serotonin 5-HT(3) receptor antagonists in mice
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Updated: Jun 25, 2026

A Modified Inflammatory Pain Model to Study the Analgesic Effect in Mice
Published on: November 15, 2024
Philippe Girard1, Yannick Pansart, Marie-Claude Coppé
1Biocodex, Service de Pharmacologie, Compiègne, France.
Background/Aims:
Post-operative nausea and vomiting are common adverse events that require administration of anti-emetic compounds, such as the serotonin 5-HT(3) receptor antagonists, but these drugs can also reduce the analgesic efficacy of some analgesics (paracetamol, tramadol).
Methods:
The present study was designed to explore the effect of 3 serotonin 5-HT(3) receptor antagonists on the antinociceptive efficacy of another frequently used post-operative analgesic, nefopam, in the mouse writhing and formalin tests.
Results:
Pre-treatment with tropisetron, ondansetron or MDL72222 did not significantly modify nefopam antinociception in both tests. However, paracetamol antinociception was blocked by ondansetron in the formalin test.
Conclusion:
These results provide a rationale for the clinical use of nefopam with anti-emetics during surgery.
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