Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Trithorax group protein ASH1 requires a combination of BAH domain and AT hooks, but not the SET domain, for mitotic chromatin binding and survival.

Chromosoma·2021
Same author

A theoretical model of Polycomb/Trithorax action unites stable epigenetic memory and dynamic regulation.

Nature communications·2020
Same author

Theoretical analysis of Polycomb-Trithorax systems predicts that poised chromatin is bistable and not bivalent.

Nature communications·2019
Same author

Noncoding RNAs in Polycomb and Trithorax Regulation: A Quantitative Perspective.

Annual review of genetics·2017
Same author

Linear ubiquitination by LUBEL has a role in Drosophila heat stress response.

EMBO reports·2016
Same author

Radical Sabbaticals.

Cell·2015

Related Experiment Video

Updated: Jun 25, 2026

Chromatin Isolation by RNA Purification (ChIRP)
11:09

Chromatin Isolation by RNA Purification (ChIRP)

Published on: March 25, 2012

Non-coding RNAs in polycomb/trithorax regulation.

Betül Hekimoglu1, Leonie Ringrose

  • 1IMBA-Institute of Molecular Biotechnology GmbH, Vienna, Austria.

RNA Biology
|March 10, 2009
PubMed
Summary

Polycomb and Trithorax proteins are epigenetic regulators. Non-coding RNAs are essential for targeting these proteins to specific gene sites, influencing gene expression and epigenetic memory.

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Developmental Biology

Background:

  • Polycomb (PcG) and Trithorax (TrxG) proteins are key epigenetic regulators.
  • They maintain gene expression patterns for developmentally crucial genes by binding to Polycomb/Trithorax Response Elements (PRE/TREs).
  • Many PRE/TREs are transcribed into long non-coding RNAs (lncRNAs).

Purpose of the Study:

  • To discuss the role of non-coding RNAs in the function of PcG and TrxG proteins.
  • To explore the molecular mechanisms underlying RNA-mediated targeting and regulation by these epigenetic factors.
  • To understand how RNA interactions contribute to epigenetic memory.

Main Methods:

  • Literature review and synthesis of current evidence.
  • Analysis of molecular mechanisms involving RNA-protein interactions.

More Related Videos

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells
11:11

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

Published on: April 25, 2018

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
10:26

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Published on: January 16, 2015

Related Experiment Videos

Last Updated: Jun 25, 2026

Chromatin Isolation by RNA Purification (ChIRP)
11:09

Chromatin Isolation by RNA Purification (ChIRP)

Published on: March 25, 2012

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells
11:11

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

Published on: April 25, 2018

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
10:26

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Published on: January 16, 2015

  • Discussion of experimental findings on non-coding RNA functions in epigenetics.

    • Main Results:

    • PcG and TrxG proteins bind to RNA.
    • RNA interactions are crucial for targeting PcG/TrxG proteins to specific genomic sites.
    • Non-coding RNAs modulate the effects of PcG and TrxG proteins on gene expression.

    Conclusions:

    • Non-coding RNAs play a critical role in recruiting PcG and TrxG proteins.
    • RNA interactions are essential for switching gene expression states.
    • These RNA-mediated mechanisms contribute to the maintenance of epigenetic memory.