Method for quantifying cutaneous pigmentation in animals and preliminary study in humans

Synopsis A preoccupation with aesthetics and a real desire for social development has brought about an increase in the demand for safe, effective depigmenting products among African and Asian populations. For many years now, we have used an experimental model which uses the black mouse CS BL/6/JICO. As a result of developing needs and the necessity to check the results obtained from animals on humans, we have had to change the methodology in order to take into account the specific characteristics of human skin. After a summary of the protocol used on the mouse, here you will find a description of the technical changes which had to be made in order to carry out the study on humans. The results of a comparative test between the two models are given. This was carried out with a cosmetic preparation containing 2% hydroquinone introduced via a vector that facilitates its absorption. The depigmentation observed in the animal model was not found significant in humans. Taking into consideration the large number of differences between individuals, and in order to demonstrate a significant result on humans, it would be necessary to use a larger experimental group, treated for a longer period than that normally used with animals.