Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Nuclei: Nuclear Spin01:08

Atomic Nuclei: Nuclear Spin

All atomic particles possess an intrinsic angular momentum, or 'spin'. Electrons, protons, and neutrons each have a spin value of ½, although protons and neutrons in nuclei may have higher half-integer spins owing to energetic factors.
Atomic nuclei have a net nuclear spin, , which can have an integer or half-integer value. In atomic nuclei, the spins of protons are paired against each other but not with neutrons, and vice versa. Consequently, an even number of protons does not contribute to...
Atomic Nuclei: Nuclear Spin State Population Distribution01:14

Atomic Nuclei: Nuclear Spin State Population Distribution

Near absolute zero temperatures, in the presence of a magnetic field, the majority of nuclei prefer the lower energy spin-up state to the higher energy spin-down state. As temperatures increase, the energy from thermal collisions distributes the spins more equally between the two states. The Boltzmann distribution equation gives the ratio of the number of spins predicted in the spin −½ (N−) and spin +½ (N+) states.
Atomic Nuclei: Nuclear Spin State Overview01:03

Atomic Nuclei: Nuclear Spin State Overview

NMR-active nuclei have energy levels called 'spin states' that are associated with the orientations of their nuclear magnetic moments. In the absence of a magnetic field, the nuclear magnetic moments are randomly oriented, and the spin states are degenerate. When an external magnetic field is applied, the spin states have only 2 + 1 orientations available to them. A proton with = ½ has two available orientations. Similarly, for a quadrupolar nucleus with a nuclear spin value of one, the...
Atomic Nuclei: Types of Nuclear Relaxation01:28

Atomic Nuclei: Types of Nuclear Relaxation

Nuclear relaxation restores the equilibrium population imbalance and can occur via spin–lattice or spin–spin mechanisms, which are first-order exponential decay processes.
In spin–lattice or longitudinal relaxation, the excited spins exchange energy with the surrounding lattice as they return to the lower energy level. Among several mechanisms that contribute to spin–lattice relaxation, magnetic dipolar interactions are significant. Here, the excited nucleus transfers energy to a nearby...
Spin–Spin Coupling Constant: Overview01:08

Spin–Spin Coupling Constant: Overview

In bromoethane, the three methyl protons are coupled to the two methylene protons that are three bonds away. In accordance with the n+1 rule, the signal from the methyl protons is split into three peaks with 1:2:1 relative intensities. The methylene protons appear as a quartet, with the relative intensities of 1:3:3:1.
Qualitatively, any spin plus-half nucleus polarizes the spins of its electrons to the minus-half state. Consequently, the paired electron in the hydrogen–carbon bond must have a...
Atomic Nuclei: Nuclear Relaxation Processes01:23

Atomic Nuclei: Nuclear Relaxation Processes

In the absence of an external magnetic field, nuclear spin states are degenerate and randomly oriented. When a magnetic field is applied, the spins begin to precess and orient themselves along (lower energy) or against (higher energy) the direction of the field. At equilibrium, a slight excess population of spins exists in the lower energy state. Because the direction of the magnetic field is fixed as the z-axis,  the precessing magnetic moments are randomly oriented around the z-axis. This...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamics of γ-aminobutyric acid concentration in the human brain in response to short visual stimulation.

Magma (New York, N.Y.)·2023
Same author

Beneficial Effects of Dinitrosyl Iron Complexes on Wound Healing Compared to Commercial Nitric Oxide Plasma Generator.

International journal of molecular sciences·2023
Same author

Spray with Nitric Oxide Donor Accelerates Wound Healing: Potential Off-the-Shelf Solution for Therapy?

Drug design, development and therapy·2022
Same author

Antagonist of M1 muscarinic acetylcholine receptor prevents neurotoxicity induced by amphetamine via nitric oxide pathway.

Annals of the New York Academy of Sciences·2008
Same author

Nitric oxide and oxidative stress in the brain of rats exposed in utero to cocaine.

Annals of the New York Academy of Sciences·2006
Same author

Memory impairments and oxidative stress in the hippocampus of in-utero cocaine-exposed rats.

Neuroreport·2005

Related Experiment Video

Updated: Jun 25, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

NO spin trapping in biological systems.

Anatoly Vanin1, Alexander Poltorakov

  • 1Semyonov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia. vanin@polymer.chph.ras.ru

Frontiers in Bioscience (Landmark Edition)
|March 11, 2009
PubMed
Summary
This summary is machine-generated.

Iron dithiocarbamate complexes effectively trap nitric oxide (NO) in plant cells. However, water-soluble complexes decompose rapidly in animal tissues, limiting their use for NO detection in animals.

More Related Videos

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
13:21

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Published on: August 18, 2012

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

Related Experiment Videos

Last Updated: Jun 25, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
13:21

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Published on: August 18, 2012

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

Area of Science:

  • Biochemistry
  • Cell Biology
  • Analytical Chemistry

Background:

  • Nitric oxide (NO) plays crucial roles in cellular signaling.
  • Iron dithiocarbamate complexes are utilized for NO detection.
  • Understanding the mechanisms of NO spin trapping is essential.

Purpose of the Study:

  • To review recent data on spin trapping of NO by iron dithiocarbamate complexes.
  • To elucidate the binding affinities and redox states involved in NO detection.
  • To assess the efficiency of these complexes in plant and animal systems.

Main Methods:

  • Analysis of iron binding affinities to dithiocarbamate ligands in the presence and absence of NO.
  • Investigation of the redox state of iron within mononitrosyl dithiocarbamate complexes.
  • Evaluation of the impact of reducing agents and superoxide on complex stability.
  • Comparison of hydrophobic and water-soluble complex performance in biological matrices.

Main Results:

  • Iron exhibits higher affinity for dithiocarbamate ligands after binding to NO.
  • Mononitrosyl iron dithiocarbamate complexes are initially Fe3+ (diamagnetic) and require reduction to become EPR-detectable.
  • Superoxide can lead to EPR-silent states, but the ABC method can mitigate this.
  • Water-soluble complexes show rapid decomposition in animal tissues, unlike hydrophobic ones.

Conclusions:

  • Iron dithiocarbamate complexes are effective for NO spin trapping in plant cells.
  • The stability and redox properties influence the detection of NO.
  • Water-soluble complexes are less suitable for NO determination in animal cells and tissues due to instability.