Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Altered motor awareness in Parkinson's disease with progressive micrographia.

Neuropsychologia·2026
Same author

Regular light-intensity exercise accelerates contextual fear extinction with reduced dorsal CA3 activation in male rats.

Neurochemistry international·2026
Same author

Dopaminergic medication alters muscle synergy during sit-to-stand motion in Parkinson's disease.

Frontiers in neurology·2026
Same author

Enigmatic Role of IL-31 in the Itch and Inflammation of Vernal Keratoconjunctivitis.

Juntendo medical journal·2026
Same author

Evaluation of LC/MS Methods for Hydrophilic Metabolites to Enable Integration of Human Blood Metabolome Data.

Mass spectrometry (Tokyo, Japan)·2026
Same author

Shifts in the timing of gait muscle synergies in older adults with reduced lower-limb motor function.

Experimental brain research·2026

Related Experiment Video

Updated: Jun 25, 2026

Analysis of Cell Cycle Position in Mammalian Cells
12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Sophisticated framework between cell cycle arrest and apoptosis induction based on p53 dynamics.

Hiroyuki Hamada1, Yoshihiko Tashima, Yu Kisaka

  • 1Laboratory for Bioinformatics, Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan.

Plos One
|March 11, 2009
PubMed
Summary

The tumor suppressor p53

More Related Videos

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Related Experiment Videos

Last Updated: Jun 25, 2026

Analysis of Cell Cycle Position in Mammalian Cells
12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Area of Science:

  • Cellular Biology
  • Biophysics
  • Systems Biology

Background:

  • The tumor suppressor p53 plays a critical role in regulating DNA repair, cell cycle arrest, and apoptosis.
  • The precise mechanisms by which p53 controls cell cycle arrest and apoptosis induction remain incompletely understood.
  • Understanding p53's regulatory role is crucial for comprehending cell fate determination and preventing malignant transformations.

Purpose of the Study:

  • To develop a novel kinetic mathematical model to investigate the influence of DNA damage on p53-mediated G2/M cell cycle arrest and intrinsic apoptosis.
  • To elucidate how DNA damage levels modulate p53 synthesis and its downstream effects on cell cycle progression and apoptosis.
  • To identify the conditions under which p53 oscillation dynamics trigger apoptosis induction.

Main Methods:

  • Construction of a novel kinetic mathematical model integrating established models to describe p53 synthesis regulation by DNA damage.
  • The model incorporates 32 dependent variables and 115 kinetic parameters to simulate cellular responses.
  • Analysis of model outputs to examine the impact of varying DNA damage levels on G2/M arrest and apoptosis.

Main Results:

  • Low DNA damage leads to a slight, sigmoidal increase in p53 synthesis over time.
  • High DNA damage induces oscillatory behavior in p53 levels.
  • Apoptosis is triggered only by severe DNA damage causing p53 oscillations and requires extreme p53 activation after G2/M arrest release.

Conclusions:

  • p53 oscillation dynamics are critical for inducing apoptosis, suggesting a key role in safeguarding against malignant transformations.
  • The cell cycle arrest system and apoptosis induction system, regulated by p53, determine cell fate.
  • This model provides a framework for understanding the dominant factors governing cell cycle arrest and apoptosis.