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Mitochondrial function after liver preservation in high or low ionic-strength solutions: a comparison between

A Y Somov1, O A Semenchenko, C J Green

  • 1Institute for Problems of Cryobiology and Cryomedicine, Kharkov, Ukraine. somov@ukr.net

Cryo Letters
|March 11, 2009
PubMed
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Modified University of Wisconsin (mod UW) and sucrose-based solution (SBS) both impact liver mitochondrial function post-preservation. SBS enhances respiration, while both solutions show inhibited ATPase activity after 24-hour cold storage.

Area of Science:

  • Organ transplantation
  • Biochemistry
  • Cellular physiology

Background:

  • Liver preservation solutions are critical for transplant success.
  • Modified University of Wisconsin (mod UW) and sucrose-based solution (SBS) are common preservation agents.
  • Understanding their impact on mitochondrial function is essential.

Purpose of the Study:

  • To evaluate mitochondrial function in liver tissue after cold storage and normothermic reperfusion.
  • To compare the effects of mod UW and SBS preservation solutions on liver mitochondria.

Main Methods:

  • Liver tissues were cold-preserved and then reperfused in vitro.
  • Assays included ATP determination, mitochondrial respiration, succinate oxidase activity, H+-ATPase activity, and intramitochondrial potassium concentration.

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  • Evaluations were performed after 1-hour and 24-hour storage periods.

    • Main Results:

    • No significant difference in ATP content after 1-hour storage, but a 50% decrease after 24-hour storage for both solutions.
    • Increased mitochondrial potassium in mod UW after 1-hour storage.
    • SBS significantly increased ADP and uncoupler-stimulated respiration, especially after 24-hour storage.
    • Succinate oxidase activity remained unchanged; H+-ATPase activity was inhibited after 24-hour storage in both groups.

    Conclusions:

    • Low ionic strength preservation solutions, like SBS, can modulate mitochondrial energy metabolism by increasing substrate oxidation.
    • Mitochondrial function is significantly affected by cold storage and reperfusion, with some changes occurring even after brief cold exposure.