Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 25, 2026

Conjunctival Commensal Isolation and Identification in Mice
07:52

Conjunctival Commensal Isolation and Identification in Mice

Published on: May 1, 2021

Contact lens-associated microbial keratitis.

Aline Silveira Moriyama1, Ana Luisa Hofling-Lima

  • 1Department of Ophthalmology, Universidade Federal de São Paulo, São Paulo, SP, Brazil. aline_moriyama@yahoo.com

Arquivos Brasileiros De Oftalmologia
|March 11, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

Microbiome of the Eye01:22

Microbiome of the Eye

The human eye has a specialized microbiota that reflects its unique anatomical and immunological environment. This low-biomass microbial community predominantly colonizes the conjunctiva and eyelid margins, playing a vital role in ocular surface homeostasis and defense. Despite its proximity to the richly colonized facial skin, the ocular surface maintains a distinct microbial profile due to continuous mechanical and biochemical defense mechanisms.The conjunctival surface hosts fewer microbial...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rose Bengal Electromagnetic Activation With Green Light for Infection Reduction: Follow-Up of a Randomized Clinical Trial.

JAMA ophthalmology·2026
Same author

Development and validation of a non-invasive RT-qPCR protocol for Nrf2 mRNA quantification in human tear fluid: A platform for ocular surface redox biomarker research.

Experimental eye research·2026
Same author

Intracameral antibiotic prophylaxis and surgical expertise: key determinants in endophthalmitis after cataract surgery.

International journal of retina and vitreous·2026
Same author

Ocular bacterial microbiome analysis by next-generation sequencing in patients with Stevens-Johnson syndrome and Sjögren's disease: associations with dry eye indices.

Experimental eye research·2025
Same author

In Vitro Evaluation of Photoactivated Rose Bengal for Growth Inhibition of Fungi Isolated From Keratitis.

Cornea·2025
Same author

Correction: Postoperative endophthalmitis treatment with antibiotics associated or not with pars plana vitrectomy: a randomized clinical trial.

International journal of retina and vitreous·2025
Same journal

Intravitreal dexamethasone sodium phosphate challenge to predict implant response in refractory diabetic macular edema: a pilot study.

Arquivos brasileiros de oftalmologia·2026
Same journal

Presumed ocular histoplasmosis syndrome phenocopy in Brazil.

Arquivos brasileiros de oftalmologia·2026
Same journal

Eyebrow and eyelid position in anophthalmic sockets reconstructed with conical or spherical implants: a quantitative analysis.

Arquivos brasileiros de oftalmologia·2026
Same journal

Corneal sensitivity recovery after phacoemulsification: quantitative esthesiometric analysis.

Arquivos brasileiros de oftalmologia·2026
Same journal

Musculoskeletal disorders in ophthalmologists and ophthalmology residents in Brazil.

Arquivos brasileiros de oftalmologia·2026
Same journal

Reliability of a Scheimpflug-based tomographer compared with a Placido-based topographer and specular microscopy in healthy eyes.

Arquivos brasileiros de oftalmologia·2026
See all related articles

Coagulase-negative Staphylococcus and Acanthamoeba were the most common causes of contact lens-related microbial keratitis. Ciprofloxacin and ofloxacin showed good susceptibility for bacterial isolates, guiding empiric treatment decisions.

Area of Science:

  • Ophthalmology
  • Microbiology
  • Infectious Diseases

Background:

  • Contact lens-associated microbial keratitis (CLAMK) is a serious sight-threatening condition with rising incidence.
  • Understanding causative agents is crucial for effective management and varies geographically.

Purpose of the Study:

  • To analyze microbiological findings in corneal scrapings from patients with CLAMK.
  • To identify common etiological agents and their susceptibility patterns.

Main Methods:

  • Retrospective analysis of patient records from January 2002 to December 2007.
  • Corneal scrapings from clinically diagnosed CLAMK cases were cultured and analyzed.

Main Results:

  • Bacterial isolates were identified in 69.46% of cases, Acanthamoeba in 39.75%, and fungi in 1.67%.

Related Experiment Videos

Last Updated: Jun 25, 2026

Conjunctival Commensal Isolation and Identification in Mice
07:52

Conjunctival Commensal Isolation and Identification in Mice

Published on: May 1, 2021

  • Coagulase-negative Staphylococcus (74 cases) and Pseudomonas spp. (32 cases) were the most frequent bacterial pathogens.
  • All coagulase-negative Staphylococcus and Pseudomonas isolates were susceptible to ciprofloxacin and ofloxacin.

    • Conclusions:

    • Coagulase-negative Staphylococcus is the predominant isolate, informing empiric treatment strategies.
    • Second-generation fluoroquinolones (ciprofloxacin, ofloxacin) and fourth-generation fluoroquinolones demonstrate good efficacy against common bacterial isolates in CLAMK.