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Related Concept Videos

Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...

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Related Experiment Video

Updated: Jun 25, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Mps1 as a link between centrosomes and genomic instability.

Christopher Kasbek1, Ching-Hui Yang, Harold A Fisk

  • 1Department of Molecular Genetics, The Ohio State University, 484 W. 12th Avenue, Columbus, OH 43210-1292, USA.

Environmental and Molecular Mutagenesis
|March 11, 2009
PubMed
Summary

Defects in Mps1 protein degradation lead to extra centrosomes, promoting genomic instability and potentially tumorigenesis. This misregulation can occur through various mechanisms, linking Mps1 to cancer development.

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Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

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Related Experiment Videos

Last Updated: Jun 25, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Centrosomes, crucial microtubule-organizing centers, must duplicate precisely before mitosis.
  • Excess centrosomes can lead to aberrant mitotic spindles, chromosome segregation errors, and genomic instability observed in human tumors.
  • Mps1 protein kinase is essential for centrosome duplication, and its degradation controls its centrosomal levels.

Purpose of the Study:

  • To investigate the role of Mps1 degradation in centrosome duplication.
  • To determine if misregulation of Mps1 degradation contributes to excess centrosome production in tumor cells.
  • To explore the link between Mps1, centrosome duplication, and genomic instability.

Main Methods:

  • Analysis of Mps1 degradation in two tumor-derived cell lines (21NT and U2OS).
  • Investigating the impact of Mps1 accumulation and non-degradable Mps1 on centrosome duplication.
  • Correlating Mps1 degradation defects with excess centrosome production during prolonged S-phase.

Main Results:

  • Mps1 degradation is misregulated in both 21NT and U2OS cell lines.
  • In 21NT cells, a mutant Mps1 accumulates at centrosomes, perturbing duplication.
  • U2OS cells express a non-degradable Mps1, causing dose-dependent centriole overduplication.

Conclusions:

  • Defects in Mps1 degradation can arise from multiple mechanisms, contributing to centrosome amplification.
  • Mps1 misregulation is implicated in the development of genomic instability and potentially tumorigenesis.
  • Mps1 acts as a critical link between centrosome duplication control and cancer progression.