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Updated: Jun 25, 2026

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
Published on: February 27, 2020
SLoMo: automated site localization of modifications from ETD/ECD mass spectra
Christopher M Bailey1, Steve M M Sweet, Debbie L Cunningham
1School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
Abstract:
Recently, software has become available to automate localization of phosphorylation sites from CID data and to assign associated confidence scores. We present an algorithm, SLoMo (Site Localization of Modifications), which extends this capability to ETD/ECD mass spectra. Furthermore, SLoMo caters for both high and low resolution data and allows for site-localization of any UniMod post-translational modification. SLoMo accepts input data from a variety of formats (e.g., Sequest, OMSSA). We validate SLoMo with high and low resolution ETD, ECD, and CID data.
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