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Published on: January 17, 2012
Molecular cloning of grass carp growth hormone receptor and its functional interaction with silver carp growth
1Shanghai Institutes for Biological Sciences, Graduate University of the Chinese Academy of Sciences, Shanghai 200233, PR China.
Abstract:
A novel grass carp growth hormone receptor (gcGHR) cDNA was cloned by RT-PCR method. CHO-K1 cells were transfected with eukaryotic expression vector pcDNA3.0 containing gcGHR entire coding region, resulting in a stable CHO cell line expressing gcGHR (CHO-gcGHR). Bioactivity assay revealed that silver carp growth hormone (scGH) bound gcGHR with dissociation constant (Kd) value of 1.96 nM and stimulated CHO-gcGHR cells proliferation with the sensitivity of 20 ng/ml. The cells proliferation assay was also provided to measure fish growth hormone levels of pituitary and refolded protein.

