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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
10:49

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

Rotavirus infections among HIV-infected children in Nairobi, Kenya

Nicholas M Kiulia1, Julia K Nyaundi, Ina Peenze

  • 1Enteric Viruses Research Group, Institute of Primate Research, Karen, Nairobi, Kenya. jmmathiu@yahoo.co.uk

Journal of Tropical Pediatrics
|March 12, 2009
PubMed

Insights

Human rotavirus (HRV) causes diarrhea in children. This study found HRV is a significant cause of diarrhea in HIV-infected Kenyan children, particularly strain G3P[6].

Area of Science:

  • Pediatrics
  • Virology
  • Infectious Diseases

Background:

  • Human rotaviruses (HRV) are a primary cause of severe diarrhea in children globally.
  • Limited data exists on rotavirus involvement in HIV-infected children's diarrhea.

Purpose of the Study:

  • To evaluate the significance of rotavirus infections in HIV-related diarrhea among Kenyan children.

Main Methods:

  • Fecal samples from 207 children were screened for HRV antigen using ELISA.
  • Positive samples underwent VP6 subgroup specificity assay, PAGE, and RT-PCR for further analysis.

Main Results:

  • 14% (29/207) of samples tested positive for rotavirus.
  • HIV-seropositive children with diarrhea showed a significantly higher prevalence of rotavirus (23.3%) compared to those without diarrhea (2.9%).
  • The predominant rotavirus strain identified was G3P[6].

Conclusions:

  • Rotavirus is a significant viral etiological agent contributing to diarrhea in HIV-seropositive children in Kenya.
  • Findings highlight the importance of considering rotavirus in the differential diagnosis of diarrhea in immunocompromised pediatric populations.

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