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Related Concept Videos

Extrinsic and Intrinsic Pathways of Hemostasis01:20

Extrinsic and Intrinsic Pathways of Hemostasis

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The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which forms a...
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Anticoagulant Drugs: Low-Molecular-Weight Heparins

Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
Formation of the Platelet Plug01:22

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Related Experiment Video

Updated: Jun 25, 2026

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae
07:37

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Published on: April 30, 2018

Progestin and thrombin regulate tissue factor expression in human term decidual cells.

C J Lockwood1, W Murk, U A Kayisli

  • 1Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

The Journal of Clinical Endocrinology and Metabolism
|March 12, 2009
PubMed
Summary

Decidual cells (DCs) express tissue factor (TF) at the maternal-fetal interface, crucial for hemostasis during childbirth. Progestin and thrombin significantly enhance TF expression in these cells.

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Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
14:55

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Published on: September 1, 2018

Area of Science:

  • Reproductive biology
  • Hemostasis and thrombosis
  • Cellular and molecular biology

Background:

  • Tissue factor (TF) initiates hemostasis via thrombin generation at the perivascular cell membrane.
  • The specific cells expressing TF and their regulation at the human maternal-fetal interface, particularly concerning hemostatic protection during normal and preterm delivery, remain unclear.

Purpose of the Study:

  • To identify TF-expressing cells within term and preterm decidual sections using immunohistochemistry.
  • To investigate the effects of progestin, thrombin, TNF-alpha, and IL-1beta on TF expression in cultured human term decidual cells (DCs).

Main Methods:

  • Immunohistochemistry was used to stain serial placental sections for TF.
  • Cultured human term decidual cells (DCs) were treated with estradiol (E2), medroxyprogesterone acetate (MPA), thrombin, TNF-alpha, and IL-1beta.
  • TF levels were quantified using ELISA, Western blotting, and quantitative real-time RT-PCR.

Main Results:

  • Decidual cells (DCs) exhibited higher TF expression at their membranes compared to villous mesenchymal cells in both term and preterm placental sections.
  • TF was not detected in interstitial or extravillous trophoblasts.
  • Medroxyprogesterone acetate (MPA) and thrombin significantly increased TF levels in cultured DCs, with a further increase when combined with estradiol (E2).
  • TNF-alpha and IL-1beta did not affect TF expression.

Conclusions:

  • Decidual cells (DCs) are a primary source of tissue factor (TF) at the maternal-fetal interface, localized to cell membranes for effective hemostasis during labor and delivery.
  • Contrary to the general understanding of constitutive TF expression, MPA and thrombin were found to significantly enhance TF expression in term decidual cells (DCs).