Identification and partial purification of a GM1-binding protein from presynaptic vesicles

Synaptic vesicles purified from rat forebrain contain a protein with membrane-binding activity. The binding has a high degree of selectivity for sialoglycosphingolipids. The protein was partially purified by affinity chromatography on lysoGM1 silica beads. The protein bands thus isolated have an apparent Mr of 65 and 53 KDa respectively.