Related Experiment Video
Updated: Jun 25, 2026

Electrophoretic Separation of Proteins
Published on: June 12, 2008
Electrophoretic separation of proteins in capillaries filled with poly(ethylene oxide)-stabilized silver
1College of Chemistry, Beijing Normal University, Beijing 100875, PR China. qinwd@bnu.edu.cn
Abstract:
Poly(ethylene oxide)-stabilized silver nanoparticles (PEO-SSNPs) were synthesized and investigated for their potential use in capillary electrophoretic separation of proteins. It was found that the PEO-SSNPs were relatively stable against changes of the buffer ionic strength, while they began to aggregate with increase of buffer pH. Adding 0.024 g/L PEO-SSNPs to 30 mmol/L phosphate buffer (pH 2.66) led to obvious increase in the peak heights of albumin bovine and albumin egg. Also, the separation efficiency for all the standards enhanced due to the suppressed wall-adsorption of proteins in the presence of the nanoparticles. The above experiments suggest that the PEO-SSNPs are effective pseudostationary phase for CE separation of proteins.
Related Concept Videos
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview
There...
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Capillary Electrophoresis: Instrumentation
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...

