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Related Concept Videos

Simple Staining Technique01:24

Simple Staining Technique

OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...

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Pathotypes of the Pinewood Nematode Bursaphelenchus xylophilus.

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Related Experiment Video

Updated: Jun 24, 2026

Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining
07:46

Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining

Published on: March 5, 2018

A rapid and simple method for staining lipid in fixed nematodes.

W T Stamps, M J Linit

    Journal of Nematology
    |March 12, 2009
    PubMed
    Summary

    This study details a new Oil Red O staining method for visualizing lipid droplets in nematode juveniles. The technique effectively stains lipids for microscopy and quantitative analysis.

    Area of Science:

    • Nematology
    • Histology
    • Biochemistry

    Background:

    • Lipid content is crucial for nematode development and dispersal.
    • Accurate visualization and quantification of nematode lipids are essential for biological studies.

    Purpose of the Study:

    • To develop and validate a reliable staining method for visualizing lipid droplets in nematode juveniles.
    • To enable quantitative analysis of lipid accumulation in Bursaphelenchus xylophilus.

    Main Methods:

    • Fixation of nematode juveniles using hot formal-acetic acid (FA4:1).
    • Staining with Oil Red O solution in ethanol at elevated temperatures.
    • Washing with ethanol and processing in glycerin for light microscopy.
    • Utilizing computerized image analysis for lipid droplet quantification.
    Keywords:
    Bursaphelenchm xylophiluslipidoil red Opine wiltpinewood nematodestaining

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    Main Results:

    • The developed method successfully stained lipid droplets within nematode juveniles as dark red spheres.
    • Lipid droplets varied in size, indicating differential lipid accumulation.
    • Computerized image analysis provided quantitative data on lipid droplet area.

    Conclusions:

    • The Oil Red O staining protocol is effective for visualizing nematode lipids.
    • This method facilitates the study of lipid metabolism and its role in nematode biology.
    • Quantitative analysis of lipid droplets can be achieved using this technique.