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Assaying Protein Kinase Activity with Radiolabeled ATP

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

TiO2-assisted silver enhanced biosensor for kinase activity profiling.

Ji Ji¹, Hui Yang, Yun Liu

¹Department of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, PR China.

Chemical Communications (Cambridge, England)

|March 12, 2009

Summary

A novel electrochemical biosensor detects protein kinase activity using titanium dioxide nanoparticles. This method enhances signal detection for kinase inhibition studies.

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Area of Science:

Biochemistry
Analytical Chemistry
Nanotechnology

Background:

Protein kinases play crucial roles in cellular signaling.
Dysregulation of protein kinase activity is implicated in various diseases, including cancer.
Sensitive detection methods are needed to study kinase activity and inhibition.

Purpose of the Study:

To develop a sensitive electrochemical biosensor for determining protein kinase activity.
To utilize the specific binding of phosphate groups to TiO2 nanoparticles for detection.
To enhance signal amplification through photo-catalytic deposition of silver nanoparticles.

Main Methods:

Development of an electrochemical biosensor platform.
Immobilization of titanium dioxide (TiO2) nanoparticles for phosphate group binding.

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Last Updated: Jun 24, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP

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Published on: May 26, 2017

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Published on: October 23, 2019

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

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Published on: June 30, 2019

Photo-catalytic deposition of silver nanoparticles (AgNPs) onto TiO2 for signal enhancement.

Electrochemical detection of protein kinase activity and inhibition.

Main Results:

The biosensor demonstrated sensitive determination of protein kinase activity.
Specific binding of phosphate groups to TiO2 nanoparticles was confirmed.
Silver nanoparticle deposition significantly enhanced the electrochemical signal.
The biosensor effectively detected kinase inhibition.

Conclusions:

The developed electrochemical biosensor offers a sensitive and efficient method for assessing protein kinase activity.
The TiO2 nanoparticle and silver nanoparticle system provides a robust platform for signal amplification.
This biosensor has potential applications in drug discovery and diagnostics for kinase-related diseases.