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Related Concept Videos

Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
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Cis-regulatory Sequences

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Related Experiment Video

Updated: Jun 24, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

Tunable c-MYC LNA i-motif.

Niti Kumar1, Michael Petersen, Souvik Maiti

  • 1Structural Biology Unit, Institute of Genomics and Integrative Biology, CSIR, Mall Road, New Delhi 110 007, India.

Chemical Communications (Cambridge, England)
|March 12, 2009
PubMed
Summary

Locked nucleic acid (LNA) nucleotides influence the formation and stability of the c-MYC DNA i-motif structure. Spectroscopic methods confirm LNA

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • The c-MYC gene is a key oncogene implicated in various cancers.
  • The c-MYC DNA sequence can form an i-motif structure, a non-canonical DNA secondary structure.
  • Modulating i-motif formation is a potential therapeutic strategy for cancer.

Purpose of the Study:

  • To investigate the impact of locked nucleic acid (LNA) nucleotides on the c-MYC i-motif structure.
  • To determine how LNA incorporation affects the stability and formation of this DNA secondary structure.

Main Methods:

  • Spectroscopic techniques were employed to analyze DNA structure.
  • The formation and stability of the c-MYC sequence with and without LNA modifications were compared.

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Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis
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Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis

Published on: October 16, 2016

Related Experiment Videos

Last Updated: Jun 24, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis
09:38

Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis

Published on: October 16, 2016

Main Results:

  • Locked nucleic acid nucleotides were shown to modulate the formation of the c-MYC i-motif.
  • LNA incorporation significantly affected the stability of the i-motif structure.

Conclusions:

  • LNA nucleotides represent a promising tool for regulating G-quadruplex and i-motif structures.
  • This study provides insights into the structural effects of LNA on oncogenic DNA sequences.