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Related Experiment Video

Updated: Jun 24, 2026

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
09:01

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Published on: March 26, 2018

Humanized model to study leukemic stem cells.

Dominique Bonnet1

  • 1Cancer Research UK, London Research Institute, Haematopoietic Stem Cell Laboratory, 44 Lincoln's Inn Fields, WC2A 3PX, London, UK. Dominique.Bonnet@cancer.org.uk

Methods in Molecular Biology (Clifton, N.J.)
|March 12, 2009
PubMed
Summary
This summary is machine-generated.

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The immunodeficient xenotransplantation model is key for studying human leukemic stem cells (LSCs). This chapter details its development, available models, and LSC purification methods, focusing on acute myeloid leukemia.

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Immunology

Background:

  • Xenotransplantation models are crucial for understanding human leukemic stem cells (LSCs).
  • These models have significantly advanced the study of leukemogenesis.
  • Acute myeloid leukemia (AML) was the first leukemia studied using this model.

Purpose of the Study:

  • To discuss the development of immunodeficient xenotransplantation models for studying LSCs.
  • To describe available immunodeficient models and their applications.
  • To outline methods for purifying LSCs.

Main Methods:

  • Utilizing immunodeficient xenotransplantation models.
  • Characterizing human leukemic stem cells within these models.
  • Purifying LSCs using established laboratory techniques.

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Last Updated: Jun 24, 2026

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
09:01

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Published on: March 26, 2018

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Main Results:

  • The xenotransplantation model has enabled the identification and characterization of LSCs.
  • Different immunodeficient models offer unique advantages for leukemia research.
  • Specific methods allow for the effective purification of LSCs.

Conclusions:

  • Immunodeficient xenotransplantation models are indispensable tools in leukemia research.
  • The choice of immunodeficient model impacts the study of leukemogenesis.
  • Purification of LSCs is a critical step for detailed characterization and therapeutic targeting.