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Related Experiment Video

Updated: Jun 24, 2026

Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy

Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy

Published on: May 8, 2020

Bdellovibrio bacteriovorus attacks Aggregatibacter actinomycetemcomitans.

M Van Essche¹, M Quirynen, I Sliepen

¹Catholic University Leuven, Department of Periodontology, Kapucijnenvoer 7, 3000 Leuven, Belgium.

Journal of Dental Research

|March 13, 2009

Summary

Predatory bacteria, Bdellovibrio bacteriovorus, effectively attacked and killed Aggregatibacter actinomycetemcomitans, a key pathogen in periodontitis. This suggests potential for B. bacteriovorus as a novel living antibiotic for treating gum disease.

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Area of Science:

Microbiology
Bacteriology
Oral Health

Background:

Periodontitis is a polymicrobial infectious disease linked to Gram-negative bacteria.
Aggregatibacter actinomycetemcomitans is a significant Gram-negative periodopathogen.
Bdellovibrio and like organisms (BALOs) are predatory bacteria targeting Gram-negative species.

Purpose of the Study:

To investigate the predatory activity of Bdellovibrio bacteriovorus against Aggregatibacter actinomycetemcomitans.
To assess the efficacy of B. bacteriovorus in reducing planktonic A. actinomycetemcomitans and its biofilms.
To explore the potential of B. bacteriovorus as a therapeutic agent for periodontitis.

Main Methods:

Challenging A. actinomycetemcomitans with B. bacteriovorus under simulated oral conditions.
Quantifying planktonic A. actinomycetemcomitans reduction using bacterial culture.
Monitoring B. bacteriovorus development via quantitative real-time PCR.
Assessing A. actinomycetemcomitans biofilm destruction using crystal violet staining and scanning electron microscopy.

Main Results:

B. bacteriovorus demonstrated significant predatory activity against A. actinomycetemcomitans.
The predator effectively reduced planktonic A. actinomycetemcomitans populations.
B. bacteriovorus successfully destroyed A. actinomycetemcomitans biofilms in vitro.
Bacterial culture and qPCR confirmed predation and predator proliferation.

Conclusions:

B. bacteriovorus can effectively attack, prey on, and kill A. actinomycetemcomitans.
These findings support the potential of B. bacteriovorus as a 'living antibiotic'.
B. bacteriovorus offers a promising avenue for novel prevention and treatment strategies for periodontitis.