Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 24, 2026

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

Controlling oligonucleotide surface density in light-directed DNA array fabrication.

Siyuan Chen¹, Margaret F Phillips, Franco Cerrina

¹Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, Wisconsin 53706, USA.

Langmuir : the ACS Journal of Surfaces and Colloids

|March 14, 2009

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Rapid Peptide Mapping of Monoclonal Antibodies with Direct Infusion Mass Spectrometry.

bioRxiv : the preprint server for biology·2026

Same author

Directed evolution of APOX for proximity labeling using phenols with high redox potentials.

Cell chemical biology·2026

Same author

Interactome screening implicates BAG6 as a suppressor of UBQLN2 misfolding in ALS/FTD.

Frontiers in molecular neuroscience·2026

Same author

DNA-damage dependent isoform switching modulates RIF1 DNA repair complex assembly and phase separation.

The Journal of biological chemistry·2026

Same author

Interactome screening implicates BAG6 as a suppressor of UBQLN2 misfolding in ALS-dementia.

bioRxiv : the preprint server for biology·2025

Same author

A needed nomenclature for nucleosomes.

Molecular cell·2025

Researchers developed a UV light method to control DNA array density for improved nucleic acid analysis. This technique enhances gene expression studies and transcription factor binding site specificity determination.

Area of Science:

Molecular Biology
Biotechnology
Genomics

Background:

High-density DNA arrays are crucial for nucleic acid analysis, impacting gene expression and SNP studies.
Oligonucleotide density on DNA arrays significantly influences hybridization thermodynamics and kinetics.
Current methods lack precise control over surface-bound oligonucleotide density.

Purpose of the Study:

To develop a method for controlling oligonucleotide density in photolithographically fabricated DNA arrays.
To improve the performance of DNA arrays for various molecular biology applications.
To offer a generalizable technique applicable to different substrate materials.

Main Methods:

Utilized a controlled UV light deprotection procedure to modulate surface synthesis sites.

More Related Videos

Simple, Affordable, and Modular Patterning of Cells using DNA

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Related Experiment Videos

Last Updated: Jun 24, 2026

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

Simple, Affordable, and Modular Patterning of Cells using DNA

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Implemented a subsequent capping reaction to inactivate exposed sites, leaving a desired fraction active.

Fabricated DNA arrays with precisely controlled oligonucleotide densities.

Main Results:

Demonstrated successful control over oligonucleotide density using UV light modulation.
Achieved lower oligonucleotide densities by controlling UV exposure and capping reactions.
Showcased the generalizability of the procedure across different substrate materials.

Conclusions:

The controlled UV deprotection and capping method effectively regulates oligonucleotide density on DNA arrays.
This technique offers enhanced precision for DNA array fabrication, benefiting applications like gene regulation studies.
The procedure's transferability to alternative substrates broadens its applicability in molecular diagnostics and research.